Table 2.
Summary of yeast two-hybrid data and choice of 8 putative Rab41/6d membrane trafficking effectors.
| (A) Synthetically defined medium selection and PCR screen | ||
|---|---|---|
| Low stringency (DDO/X/A) | 593 hits | |
| High stringency (QDO/X/A) | 265 hits | |
| After duplicate elimination by PCR/HaeIII digestion | 155 hits (sequenced using single primer) | |
| (B) 8 putative Rab41/6d membrane trafficking effectors identified by sequence and database comparisions | ||
| Protein name | UniProtKB Accession No. | Putative functions |
| Syntaxin 8 | Q9UNK0.2 | Vesicle trafficking protein that functions in the early secretory pathway, possibly by mediating retrograde transport from cis-Golgi to the ER |
| Dynactin 6 | O00399.1 | Antigen processing and presentation of exogenous peptide antigen via MHC class II; mitotic spindle organization |
| Kif18A | Q8NI77.2 | Chromosome congression |
| Endofin | Q7Z3T8.3 | May be involved in regulating membrane trafficking in the endosomal pathway |
| Adaptor protein complex AP-1 subunit gamma-1 | O43747.5 | Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network and/or endosomes |
| Adaptor-related protein complex 3 subunit mu-1 | Q9Y2T2.1 | Part of the AP-3 complex. The complex facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes |
| Rabconnectin-3 | Q8TDJ6.2 | May serve as a scafford protein for both Rab3 GEF and GAP on synaptic vesicles |
| Selenium-binding protein 1 | Q13228.2 | May be involved in intra-Golgi protein transport |