Table 3.
Quantification of Golgi fragmentation.
| Protein targeted | siRNA | Number of Golgi fragments | Normalized number of Golgi fragments |
|---|---|---|---|
| Control | Control siRNA | 3.10 ± 0.24 | 1.0 |
| Rab41/6d | Rab41/6d siRNA | 34.45 ± 10.39 | 11.1 |
| Kif18A | Kif18A siRNA (3) | 4.21 ± 0.35 | 1.4 |
| Kif18A siRNA (4) | 3.79 ± 1.41 | 1.2 | |
| Syntaxin 8 | Syntaxin 8 siRNA (3) | 14.17 ± 3.54 | 4.6 |
| Dynactin 6 | Dynactin 6 siRNA (2) | 11.28 ± 3.03 | 3.6 |
| Dynactin 6 siRNA (3) | 27.58 ± 8.05 | 8.9 |
Quantification of Golgi fragmentation was determined using deconvolved images to sharpen the distinction between Golgi apparatus and general cytoplasmic fluorescence. Deconvolved images were then segmented between Golgi fragments and general cytoplasm based on the intensity of GalNAcT2-GFP fluorescence, and the number of Golgi fragments was determined using iVision-MAC software. ~30 cells were analyzed for each data point. Data are shown ± standard error of the mean. Bolded data values emphasize the strong Golgi fragmentation with these conditions.