Figure 1.

Workflow of transcriptome sequencing for four parents. (i) Leaves and floral buds of the four gerbera genotypes used to isolate RNA; (ii) Mixed cDNA libraries of four genotypes sequenced on an Illumina platform; (iii) Raw reads used for pre-processing to trim adapter sequences and to filter reads with low quality; (iv) Transcripts of SP1 assembled as first step toward reference transcriptome construction; (v) Transcripts of other genotypes mapped to reference to yield consensus contigs named “Cap3Contigs_All” for SNP detection and annotation.