Figure 4.

Antitumor Th1-type cytokine production in the liver and liver-draining lymph nodes (LDLN) from Salmonella-treated mice. Twenty-one days after treatment with CVD 915 or PBS, livers and LDLN from tumor-bearing mice were removed. Cytokine production was measured by ELISA in 96 h culture supernatants of intrahepatic immune cells (IHIC) and LDLN cells following exposure to LM3 tumor or not. (A) IFN-γ, (B) TNF, and (C) IL-2. The limits of detection were 30, 4, and 4 pg/ml, respectively. Samples from four to five animals per group were pooled. Data shown are mean values of triplicate or quadruplicate cultures and are representative of two independent experiments. ns, not statistically significant, *p < 0.05, **p < 0.001, ***p < 0.0001.