FIGURE 1.

Adhesion of L. reuteri to HT-29 cells is host strain-specific. HT-29 cells were incubated with L. reuteri or EPEC for 1 h, and adherent bacteria were quantified by CFU (A) and/or visualized by immunofluorescence staining (B). (A) Adhesion is expressed as percentage of adherent bacteria relative to the inoculum. Data are shown as means ± SEM from three independent experiments performed in duplicate. (B) L. reuteri ATCC PTA 6475 (left) and 100-23C (right) were stained with anti-CmbA and anti-SRR (red), respectively. Cell nuclei were counterstained with DAPI in blue. Shown are representative images from three independent experiments performed in duplicate. Bar = 10 μm.