Figure 2.
Heterozygous bumble mice have fewer IgM-secreting B cells. Cell suspensions in serial dilutions were plated on ELISpot plates coated with anti-IgM. IgM antibody-secreting cells (ASC) in (A) spleen and (B) bone marrow. Upper panels display IgM ASCs per 106 cells and lower panels indicate mean spot size. Spot size is influenced by the number of spots per well. Therefore, wells containing similar numbers of spots across groups were used to calculate spot size (range 10–30 spots for bone marrow and 30–70 for spleen). Data were compiled from two experiments.