Table 3.

Mean cell counts (cells mm²) of immunohistochemistry for apoptosis (activated caspase‐3; Cas‐3), inflammation (ionised calcium‐binding adapter molecule 1; IBA‐1) and astrogliosis (glial fibrillary acidic protein; GFAP)

	Control	Control+UCB	Asphyxia	Asphyxia+UCB	P
Activated Cas‐3
Hippo: CA1	330 ± 80*	258 ± 103	708 ± 85†, ‡	197 ± 84*	< 0.01
Hippo: DG	827 ± 160*	506 ± 185*	1401 ± 72†, §, ‡	559 ± 131*	< 0.01
SCWM	156 ± 56*	80 ± 71*	537 ± 67†, §, ‡	206 ± 114*	0.02
Striat: IC	169 ± 83*	49 ± 38*	438 ± 56†, §, ‡	62 ± 18*	< 0.01
Thal: PrT	192 ± 78	159 ± 84	372 ± 47‡	49 ± 14*	< 0.01
Cortex: PM	158 ± 66*	126 ± 32	462 ± 87†, ‡	163 ± 35*	<0.01
IBA‐1
Hippo:CA1	55 ± 18*	65 ± 26*	356 ± 57† § ‡	122 ± 53*	< 0.01
Hippo: DG	119 ± 46*	84 ± 24*	387 ± 53†, §, ‡	162 ± 67*	< 0.01
SCWM	139 ± 43*	146 ± 33	319 ± 44†	168 ± 42	0.02
Striat: IC	177 ± 35*	179 ± 74	332 ± 27†, ‡	186 ± 53*	< 0.01
Thal: PrT	44 ± 27*	125 ± 30*	449 ± 57†, §, ‡	179 ± 42*	< 0.01
Cortex: PM	72 ± 22	122 ± 44	227 ± 49	101 ± 56	0.08
GFAP
Hippo: CA1	256 ± 83*	174 ± 79*	701 ± 57†, §, ‡	226 ± 103*	< 0.01
Hippo: DG	437 ± 129	217 ± 124	758 ± 55	367 ± 138	< 0.01
SCWM	326 ± 83	189 ± 179*	609 ± 91§, ‡	167 ± 106*	0.01
Striat: IC	415 ± 127	244 ± 137	660 ± 45‡	219 ± 79*	< 0.01
Thal: PrT	410 ± 111*	415 ± 189	760 ± 66†, ‡	334 ± 101*	< 0.01
Cortex: PM	130 ± 67*	67 ± 23	479 ± 79†, ‡	74 ± 44*	< 0.01

There was a significant increase in apoptosis, inflammation and astrogliosis in all regions examined except for inflammation in the cortex. Hippo: CA1, hippocampus: CA1; Hippo: DG, hippcampus: dentate gyrus; SCWM, subcortical white matter; Striat: IC, striatum: internal capsule; Thal: PrT, thalamus: pretectal nucleus; Cortex: PM, cortex: pleomorphic layer.

P < 0.05: ^† vs. control; ^§ vs. control+UCB; *vs. asphyxia; ^‡ vs. asphyxia+UCB.