Fig 2. Cyclic AMP levels in prepubertal and adult oocytes treated with or without cAMP modulators prior to and during IVM.

Data are presented as mean ± SEM (n = 3). Optical density reads from ELISA test were analyzed using two-way ANOVA. Bars labeled with different superscripts represent statistical significance among treatments (a, b); P < 0.05. Oocytes were retrieved via OPU. The cAMP modulators increased intra-oocyte cAMP levels in both prepubertal and adult oocytes after pre-IVM (P < 0.05). No differences in cAMP profiles were found among treatments after IVM (P > 0.05).