Fig 4. Gene expression profiles in expanded blastocysts produced from adult and prepubertal oocytes treated with or without cAMP modulators prior to and during IVM.

Data are shown as the mean ± SEM (n = 12). Data were analyzed using two-way ANOVA followed by a Tukey's range test. The asterisk represents statistical significance among treatments for the same transcript; P < 0.05. In vivo produced expanded blastocysts were used for comparison. The mRNA relative abundance of the EGR1 gene was lower in all in vitro derived blastocysts compared to in vivo produced counterparts. No differences among treatments were found for DNMT3b, BCL2L1, PRDX1, SLC2A8.