Table 2. In vitro and in vivo developmental rates of prepubertal and adult oocytes cultured pre- and during IVM with or without cAMP modulators.
| Donors | Treatment | Replicates (n) | Total retrieved oocytes | Total IVM oocytes | Cleavage rates/IVM oocytes (%)* | Blastocysts rates/IVM oocytes (%)* | Embryo transfer (n) | Pregnancy at 45 d, n(%) | Pregnancy at 90 d, n(%) | Pregnancy at 180 d, n(%) |
|---|---|---|---|---|---|---|---|---|---|---|
| Prepubertal | cAMP30 | 18 | 352 | 217 | 57.1±5.2 | 18.3±2.8 ab | 17 | 7(41.2) | 6(35.3) | 5(29.4) |
| DMSO30 | 18 | 406 | 222 | 49.5±3.3 | 13.7±2.0b | —— | —— | —— | —— | |
| TCM24 | 18 | 306 | 208 | 62.0±3.6 | 23.3±5.1 ab | —— | —— | —— | —— | |
| Adult | cAMP30 | 18 | 257 | 162 | 58.8±5.4 | 26.5±3.0a | 15 | 9(60) | 7(46.7) | 7(46.7) |
| DMSO30 | 18 | 271 | 190 | 51.1±4.2 | 20.3±2.9ab | —— | —— | —— | —— | |
| TCM24 | 18 | 257 | 206 | 53.0±5.5 | 25.9±3.3a | —— | —— | —— | —— |
* Data are the mean ± SEM.
Oocyte retrieval was performed via OPU. Different superscripts indicate statistical significances among treatments groups (a, b); P < 0.05. Lower blastocyst yields were found for oocytes treated with the DMSO30 protocol. Similar pregnancy rates were observed for blastocysts produced from prepubertal and adult donors using the cAMP30 treatment.