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Representative 99mTc-Annexin V images of methylprednisolone-induced femoral head necrosis. (a) 99mTc-Annexin V image of a saline-treated rabbit as control. (b) 99mTc-Annexin V image of a rabbit after 8 weeks of prednisolone treatment. Radioactivity accumulates in the femoral head regions as indicated by the arrows. (c) TUNEL assay of a femoral head section, 8 weeks after prednisolone administration, with apoptotic cells stained brown, as indicated by the arrow.
