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. 2016 Feb 16;2016:5868913. doi: 10.1155/2016/5868913

Figure 1.

Figure 1

Expression of left ventricle p47phox and p67phox in Sed-control, Sed-Dia, ExT-control, and ExT-Dia animals. (a) RT-PCR of p47phox: top: a representative gel; bottom: quantification of p47phox transcript normalized to β-actin as loading control. n = 10 in each group. (b) Western blot analysis of left ventricle p47phox in four groups: top: a representative gel; bottom: bar graph of summary data of densitometry analyses of p47phox protein level normalized to actin for loading variations. n = 6–8 in each group. Values represent mean ± S.E. (c) RT-PCR of left ventricle p67phox in Sed-control, Sed-Dia, ExT-control, and ExT-Dia animals: top: a representative gel; bottom: quantification of p67phox transcript normalized to β-actin as loading control. n = 6 in each group. (d) Protein expression of p67phox in four groups: top: a representative Western blot; bottom: densitometry analysis of p67phox protein normalized to β-actin as loading control. n = 3-4 in each group. Values are represented as mean ± S.E. ∗ P < 0.05 versus Sed-control. + P < 0.05 versus Sed-Dia group.