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. 2016 Jan 25;14(2):20. doi: 10.3390/md14020020

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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(+)-Obtusol increases ROS level in the larvae. Second instar Aedes aegypti larvae (Rockefeller and wild strains), were incubated in water under different conditions; (A) bright field image of larvae incubated in distilled water; (B) fluorescent control image of larvae incubated in distilled water alone; (C) bright field image of larvae incubated in distilled water containing 0.1% DMSO; (D) fluorescent control image of larvae incubated in distilled water containing 0.1% DMSO; (E) bright field image of second instar larvae (Rockefeller strain), incubated with water containing 5 ppm (+)-obtusol; (F) fluorescent image of second instar larvae (Rockefeller strain), incubated with water containing 5 ppm (+)-obtusol; (G) bright field image of second instar larva (wild strain), incubated in water containing 5 ppm (+)-obtusol; (H) fluorescent image of second instar larva (wild strain), incubated in water containing 5 ppm (+)-obtusol. Scale bars: 100 µm.

(+)-Obtusol increases ROS level in the larvae. Second instar Aedes aegypti larvae (Rockefeller and wild strains), were incubated in water under different conditions; (A) bright field image of larvae incubated in distilled water; (B) fluorescent control image of larvae incubated in distilled water alone; (C) bright field image of larvae incubated in distilled water containing 0.1% DMSO; (D) fluorescent control image of larvae incubated in distilled water containing 0.1% DMSO; (E) bright field image of second instar larvae (Rockefeller strain), incubated with water containing 5 ppm (+)-obtusol; (F) fluorescent image of second instar larvae (Rockefeller strain), incubated with water containing 5 ppm (+)-obtusol; (G) bright field image of second instar larva (wild strain), incubated in water containing 5 ppm (+)-obtusol; (H) fluorescent image of second instar larva (wild strain), incubated in water containing 5 ppm (+)-obtusol. Scale bars: 100 µm.