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. 2016 Feb 4;14(2):28. doi: 10.3390/md14020028

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) HIV infection of RIC cells: Three biological replicates of 11 HPLC fractions (approx. 20 μg/mL each) generated from S. carteri SPE F1 were tested for the ability to inhibit HIV-1 replication in the EASY-HIT assay; (B) the cell viability of the infected LC5-RIC reporter cells treated with the three biological replicates of HPLC fraction 1–11 generated from S. carteri SPE F1 were assessed by the Microculture Tetrazolium Test (MTT). The error bars represent one standard deviation from the mean. p values from unpaired t-tests between the neighboring HPLC fractions are as follows for % infection and % cell viability: p = 0.43, 0.09 (HPLC 1:2), p = 0.0005, 0.0004 (HPLC 2:3), p = 0.8289, 0.8790 (HPLC 3:4), p = 0.1125, 0.1500 (HPLC 4:5), p = 0.0315, 0.4033 (HPLC 5:6), p = 0.2341, 0.0209, (HPLC 6:7), p = 0.3528, 0.3941 (HPLC 7:8), p = 0.3464, 0.5351 (HPLC 8:9), p = 0.7839, 0.0413 (HPLC 9:10), p = 0.9877, 0.0892(HPLC 10:11).