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. 2016 Mar;26(3):315–330. doi: 10.1101/gr.195248.115

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© 2016 Sheu et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Proximal segment of the Mcm4 NSD delays late origin firing in HU when checkpoint control on Sld3 and Dbf4 is relieved. Yeast cells were synchronized in G1 phase and released into YPD containing 0.2 M HU and 0.5 mM EdU. (A) Replication profiles of Chromosome IV for WT and the mcm4^Δ74-174 sld3-38A dbf4-19A triple mutant at 25, 50, and 75 min after release into HU from G1 arrest. (B) Replication profiles of Chromosome IV for the sld3-38A dbf4-19A and mcm4^Δ74-174 sld3-38A dbf4-19A mutants at 25, 50, and 75 min after release into HU from G1. (C) Distribution of fork progression from activated origins at the indicated time point for the wild type (blue, data from A), sld3-38A dbf4-19A (orange, data from B), and mcm4^Δ74-174 sld3-38A dbf4-19A (red; left panels, data from A; right panels, data from B) mutants. Box graph shows the fork progression, excluding peaks with heights <10% of the maximal height scale.