Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar;26(3):365–375. doi: 10.1101/gr.196857.115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Peace et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 4. — Fkh1-OE increases early origin firing and decreases replication fork rate. Strains JPy88 (WT) and JPy89 (Fkh1-OE) arrested and induced as described in Figure 2A were released into S phase without HU, and aliquots were incubated with BrdU for the indicated intervals and harvested. (A) qBrdU-seq analysis was performed in duplicate and plotted for Chromosome XI. (B) Analysis of the earliest decile of origins: Average BrdU incorporation is plotted for the indicated time intervals (upper panel), and the horizontal dashed line indicates the position at which fork rate measurements were calculated; the difference in BrdU incorporation between the first two intervals is plotted (lower panel). (C) Quantification of BrdU maximum peak height for the indicated loci for each time interval. Total BrdU (summed max peak heights) includes all origins including all copies of 2-micron and rDNA (ARS1200) origins. (D) Immunoblot analysis of Rad53; Rad53 is indicated with an asterisk, and phosphorylated Rad53 is indicated with two asterisks. “MMS” indicates sample was released after G1 block and Gal-induction into the presence of 0.033% MMS for 1 h prior to harvest.