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. 2016 Feb 19;8(2):102. doi: 10.3390/nu8020102

Figure 2.

Figure 2

Fto affected 3T3-L1 cell proliferation. 3T3-L1 cells were transfected with Fto siRNA and NC siRNA (A), or transfected with Fto expression plasmid and vacant plasmid pcDNA3.1 (B) for 24 h. Cells were seeded in 24-well plates and were counted at Day 1 to Day 7, respectively. Cell viability was expressed as the percentage of viable cells relative to cell viability detected in the mock transfected control cells. All samples were detected in triplicate. * p < 0.05, versus control. (C) 3T3-L1 cells were transfected with siRNA and plasmid for 24 h, and after adipogenic induction for another 24 h, then the cell proliferation was analyzed via EdU incorporation. The representative images were shown as above; EdU (Red), Hoechest 33342 (blue); scale bar, 50 μM. (D) Quantification analysis of EdU positive cells.