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. 2016 Feb 19;8(2):102. doi: 10.3390/nu8020102

Figure 3.

Figure 3

Fto influenced the mitochondrial membrane potential and ATP generation of pre-adipocytes. (A,B) Mitochondrial membrane potential of 3T3-L1 cells were determined using JC-1, 24 h after transfection. The ratio of aggregated JC-1 and monomeric JC-1 represented Δψm of cells. Results are expressed as the means ± SD from three independent experiments. * p < 0.05 versus NC/empty pcDNA3.1 vector group. (C,D) Cellular ATP concentrations were detected in 3T3-L1 cells 24 h after transfection. Results are expressed as the means ± SD from 3 independent experiments. * p < 0.05 versus NC/empty pcDNA3.1 vector group. (E) Parental and mock transfected control 3T3-L1 cells, together with Fto-deficient or Fto-overexpressed pre-adipocytes were incubated with MitoTracker Red CM-H2XRos staining, 24 h after transfection. The images were visualized by fluorescence microscopy as shown above; scale bar, 20 μm. (F) Quantification analysis of MitoTracker fluorescence intensity.