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CheA phosphotransfer reactions. After CheA autophosphorylation, the phosphate group can be transferred from the P1 histidine residue to CheY or back to ADP or undergo hydrolysis. Bimolecular binding of ATP to CheA is thought to be fast relative to autophosphorylation under conditions of excess ATP.75 Under steady-state conditions, when ATP and CheY are in excess and phosphotransfer from CheA-P to CheY is relatively fast, [CheY-P] = k1/k–p[CheA].
