Table 1. Tar_FO Constructs Generated.

	methylation state	N- or C-foldon	first Tar KCM (residue numbers)	second Tar KCM (residue numbers)
1	QEQE	n/a	257–521	257–521
2	QEQE	N	257–521	257–521
3	QEQE	N	263–515	263–553
4	QEQE	N	263–515	263–515
5	QEQE	N	GGGGG-263–515	263–515
6	QEQE	N	263–515	263–515-NWETF
7	QEQE	C	GGGGG-263–515	263–515
8	QEQE	C	263–515	263–515
9	QEQE	C	263–515	263–521
10	QEQE	C	257–521	263–515
11	QEQE	C	257–521	257–521
12	QEQE	C	257–521	257–515
13	QQQQ	n/a	257–521	257–521
14	QQQQ	C	257–521	257–515
15	QQQQ	C	257–521	257–521
16	QQQQ	C	257–521	257–528
17	n/a	C	353–424-GSAGTSG	353–424-GASGSTG