Table 2. Kinetic Parameters for Autophosphorylation Assaysa.
components | A0 (CheA-P relative) | k1 (min–1) | k1A0 (CheA-P min–1) | R2 |
---|---|---|---|---|
CheA and CheW | 0.99 ± 0.01 | 1.18 ± 0.05 | 1.2 | 0.99 |
CheA, CheW, and TarSC 4Q | 8 ± 1 | 0.09 ± 0.02 | 0.7 | 0.98 |
CheA, CheW, and TarFO 4Q | 6.7 ± 0.9 | 0.20 ± 0.06 | 1.3 | 0.95 |
CheA, CheW, and TarFO short | 18 ± 1 | 0.073 ± 0.009 | 1.3 | 0.98 |
Prefactor and rate constant values from activity vs time fits to a first-order expression (see Experimental Procedures).