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. 2016 Mar 1;6:22071. doi: 10.1038/srep22071

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Copyright © 2016, Macmillan Publishers Limited

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(a) Confocal fluorescence images of living SH-SY5Y cells labeling nuclei by Hoechst 33342 (blue) and mitochondria by MitoTracker Green FM (green) and incorporating quantum dots (red). Scale bar represents 20 μm. (b) Representative time-lapse of fluorescence ratio (upper) and the converted temperature change (lower) of a single quantum dot. Chemical stimulation by 10 μM CCCP was applied at 130 sec. (c) Summary of fluorescence ratio response before (left) and after (right) CCCP stimulation. . The number of samples of which the change in fluorescence intensity ratio before and after the stimulus was higher than the resolution and increase (red), in the range of the resolution (black), and higher than the resolution and decrease (blue) was 5, 1, and 4, respectively. Fluorescence intensity ratios were calibrated by estimated diameter. (d) Summary of temperature change for each measurement. Red lines present the resolution of temperature.

Inline graphic — (a) Confocal fluorescence images of living SH-SY5Y cells labeling nuclei by Hoechst 33342 (blue) and mitochondria by MitoTracker Green FM (green) and incorporating quantum dots (red). Scale bar represents 20 μm. (b) Representative time-lapse of fluorescence ratio (upper) and the converted temperature change (lower) of a single quantum dot. Chemical stimulation by 10 μM CCCP was applied at 130 sec. (c) Summary of fluorescence ratio response before (left) and after (right) CCCP stimulation. . The number of samples of which the change in fluorescence intensity ratio before and after the stimulus was higher than the resolution and increase (red), in the range of the resolution (black), and higher than the resolution and decrease (blue) was 5, 1, and 4, respectively. Fluorescence intensity ratios were calibrated by estimated diameter. (d) Summary of temperature change for each measurement. Red lines present the resolution of temperature.