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. 2016 Mar 1;6:22275. doi: 10.1038/srep22275

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Copyright © 2016, Macmillan Publishers Limited

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Tumor tissues were obtained from the uterine cervix by punch biopsy 30 min after the initial dose of irradiation. Tissues were stained with 53BP1 and DAPI and visualized using an advanced high-resolution microscopy technique with deconvolution. (a) Representative images of 53BP1 foci in a carbon ion-irradiated specimen. (b) Enlarged image of the area indicated by the white box in (a). (c) Representative images of 53BP1 foci in an X-ray-irradiated specimen. (d) Enlarged image of the area indicated by the white box in (c). (e,f) Polygon images of a carbon ion- (e) and X-ray- (f) irradiated cell. The 3D polygon image of 53BP1 foci was created using Imaris 8.0.1.