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. 2016 Mar 1;6:22325. doi: 10.1038/srep22325

Figure 5. NAD+ promotes skin allograft survival through a systemic increase of IL-10.

Figure 5

Fully MHC-mismatched C57BL/B6 tail skin allografts were transplanted onto DBA/2 mice that received daily doses of NAD+ (40 mg in 100 μl PBS) or control solution (PBS). (a) Skin graft survival was monitored (n = 6 per group) and (b) CD4+ T cells were isolated from spleens of wild type mice 8 days after transplantation and frequencies of IL-10+, IL-17A+, and IFNγ+ cells were analyzed by flow cytometry (n = 6 per group, representative plots shown). (c) Fully MHC-mismatched DBA tail skin allografts were transplanted onto IL-10−/−(C57BL/6 background), CD4−/− (C57BL/6 background), (and wild type (WT) mice that received daily doses of NAD+ (40 mg in 100 μl PBS) or control solution (PBS) and skin graft survival was monitored (n = 6 per group). Data derived from two independent experiments. Data represent mean ± s.d. *P < 0.05; **P < 0.01; ***P < 0.001. Student’s t-test, ANOVA tests and Log-rank test were used to compare groups accordingly.