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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Hum Mutat. 2011 Dec 20;33(2):440–447. doi: 10.1002/humu.21662

Figure 2. Effect of missense variants on SCRIB-VANGL2 interactions by Co-Ip.

Figure 2

(a) Specific interaction of SCRIB with VANGL2 is through the PDZ binding domain. Western blotting of the lysate with anti-FLAG and anti-HA demonstrates the presence of FLAG-SCRIB and HA-VANGL2 proteins respectively. Following immunoprecipitation with anti-FLAG, full-length VANGL2 is pulled down but VANGL2Δ (lacking PDZ binding domain) is not. (b) A similar experiment was conducted with full-length HA-VANGL2 and FLAG-SCRIB constructs with various missense changes or wild type sequence. None of the variants, including the mouse pathogenic change I285K affect the ability of SCRIB to pull down VANGL2.