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. 2016 Mar 1;6:22372. doi: 10.1038/srep22372

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Copyright © 2016, Macmillan Publishers Limited

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Panel (A) demonstrates NagZ activity on peptidoglycan. Gonococci were metabolically labeled with [6-³H]glucosamine. After a chase period, supernatants were collected and treated with purified NagZ. Radiolabeled fragments were separated by size exclusion chromatography and detected by liquid scintillation counting. Gonococcal peptidoglycan contains PG dimers, monomers, free disaccharide, and anhydro-N-acetylmuramic acid. GlcNAc, N-acetylglucosamine; MurNAc, N-acetylmuramic acid; anhMurNAc, anhydro-N-acetylmuramic acid. Panel (B) is an SDS-PAGE gel of LOS isolated from: 1) N. gonorrhoeae F62 Δ8-1⁴²; 2) N. gonorrhoeae F62; 3) N. gonorrhoeae F62ΔNagZ; and 4) N. gonorrhoeae F62ΔNagZ treated with NagZ. The lower portion of the panel is a graphical representation of the oligosaccharide portion of LOS isolated from F62⁵¹. Letters above the arrows correspond to the LOS bands seen in the upper panel.