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. 2016 Mar 1;6:22380. doi: 10.1038/srep22380

Figure 2. Expression difference of pluripotency related miRNAs in cells and cell culture mediums of fully and partially pluripotent stem cells.

Figure 2

(A) Relative expression levels of miRNA-323-3p in cell culture mediums of partially pluripotent iPSCs (IP20D-3 and IP36D-3), fully pluripotent iPSCs (IP14D-1, IP14D-6, IP16DT-2A, IP14DN-5) and ESCs (ESC2 and CL11), evaluated by real-time PCR. (B) Relative expression levels of miRNA-323-3p in cells evaluated by real-time PCR. (C) Relative expression levels of miRNA-292-3p and miRNA-294 in cell culture mediums evaluated by real-time PCR. (D) Relative expression levels of miRNA-292-3p and miRNA-294 in cells evaluated by real-time PCR. *P < 0.05, **P < 0.01, ***P < 0.001, compared to IP20D-3, #P < 0.05, ##P < 0.01, ###P < 0.001, compared to IP36D-3, the Student’s t-test. All the data represent three independent experiments. (E) Relative expression levels of miRNA-323-3p in eight iPS clones and ESCs cell culture mediums evaluated by real-time PCR. 2N represents partially pluripotent stem cells and 4N represents fully pluripotent stem cells. **P < 0.01, compared to ESC2, the Student’s t-test. All the data represent three independent experiments.