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. 2016 Mar 1;6:22380. doi: 10.1038/srep22380

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Copyright © 2016, Macmillan Publishers Limited

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(A) Relative expression levels of miRNA-292-3p, miRNA-294, miRNA-7-5p and miRNA-9-3p in cell culture mediums along the neuronal differentiation process evaluated by real-time PCR. (B) Immunostaining of TUJ1 and PITX3 protein in differentiated cells at day 40. Expression of TUJ1 (red) and PITX3 (green) proteins was detected. DNA was stained by Hoechst 33342 (blue). (C) Relative expression levels of miRNA-292-3p, miRNA-294, miRNA-499a-5p and miRNA-1-5p in cell culture mediums along the cardiomyocyte differentiation process evaluated by real-time PCR. (D) Immunostaining of cTnT protein in differentiated cells at day 22. Positive cTnT (green) was observed, DNA was stained by Hoechst 33342 (blue). (E) Correlation analysis of miRNA-7-5p relative expression in cell culture mediums and the percentage of TUJ1 positive cells at day 30, day 35, day 40 and day 45 during neuronal differentiation process. (F) Correlation analysis of miRNA-499a-5p relative expression in cell culture mediums and the percentage of cTnT positive cells at day 12, day 15, day 18 and day 21 during cardiomyocyte differentiation process.