Fig. 5. G5-7 suppresses tumor growth of U87MG/EGFRvIII cells and elongates nude mouse life span.

(A) Survival curves of brain tumor–bearing mice. After confirmation of brain tumor formation by MRI, mice were orally treated with vehicle or G5-7 once a day until the termination of the experiment. The experiments were repeated three times (n = 9 to 10 mice per group). (B) Quantitative analysis of intracranial tumor volume in the mice treated with or without G5-7 (n = 6 to 8 per group). Data are means ± SEM (*P < 0.05, one-way ANOVA, n = 3). (C) Western blot analysis of intracranial tumor lysates from the animals treated with G5-7 (10 and 50 mg/kg) or vehicle for 21 days. Blots are representative of three experiments. (D and E) Analysis of proliferation staining for Ki67 (D) and apoptosis using the TUNEL assay (E) in tumor nodules from vehicle- or G5-7–treated mice. Scale bars, 50 μm. Data are means ± SEM (*P < 0.05, **P < 0.01, two-tailed Student’s t test, n = 3).