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. 2016 Mar 1;6:22403. doi: 10.1038/srep22403

Table 4. Assignment of control factors and levels in the nested experimental design using an orthogonal matrix (L9 (34)).

Factors Sonication time (min) Sonication temperature ( °C) Output power (W) Seed soaking time (h)
Level 1 15 25 200 1
Level 2 35 45 350 5
Level 3 55 65 500 9