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. 2015 Sep 10;19(1):pyv083. doi: 10.1093/ijnp/pyv083

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© The Author 2015. Published by Oxford University Press on behalf of CINP.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Peroxisome proliferator-activated receptor δ (PPARδ) overexpression promoted proliferation and differentiation of neural stem cells (NSCs) in vitro. (A) Shown is the protein marker nestin, expressed by NSCs. (B) The coding sequence of mouse PPARδ (LV-PPARδ) increases proliferation determined by 3-(4, 5-dimethythiazole-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) and cell counting kit (CCK-8) assays (n = 6). (C) Representatives of bromodeoxyuridine positive (BrdU⁺)-labeled cells of the NSCs after treatment with a lentiviral vector that selectively expresses PPARδ with enhanced green fluorescent protein (LV-PPARδ-EGFP). (D) Statistical graph shows the number of BrdU⁺ cells in different groups (n = 6). (E) Immunocytochemistry for neuronal marker neuronal nuclear antigen (NeuN) was used to assess neural differentiation in NSCs treated with LV-PPARδ. (F) Shown are statistical data of NeuN⁺-positive neurons in different groups (n = 6). Data are mean ± standard error of the mean. ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, compared with control; ^# p < 0.001, compared with a lentiviral vector expressing EGFP alone (LV-EGFP). Scale bars = 50 μm.