Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 29;19(1):pyv085. doi: 10.1093/ijnp/pyv085

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2015. Published by Oxford University Press on behalf of CINP.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — Effect of ER272 (1) on neural progenitor cell (NPC) proliferation. (A) Phase contrast microscopy images of neurosphere cultures stimulated with basic fibroblast growth factor (bFGF) in the absence and presence of 1 µM ER272 (1). Calibration bar indicates 200 µm. (B) Effect of different concentrations of ER272 (1) on neurosphere area. (C) Effect of different concentrations of ER272 (1) on neurosphere number. (D) Effect of 1 µM ER272 (1) on cell viability assayed by trypan blue exclusion. (E) Fluorescence microscopy images of NPC cultures stimulated with bFGF in the presence and absence of 1 µM ER272 (1) and/or 0.5 µM of the protein kinase C (PKC) inhibitor G06850. Cells were immunostained for Ki67 detection and nuclei were counterstained with 4',6-diamidino-2-phenylindole. (F) Quantification of Ki67⁺ cells in cultures described in E. *P<.05 compared with control in a Student’s t test; #P<.05 compared with the other groups by 1-way ANOVA.