Table 2.

Model differential equations

Reaction	Flux
PI to PI(4)P	k_4K * [PI] − k_4P * [PIP]
PI(4)P to PI(4,5)P2	k_5K * [PIP] − k_5P * [PIP2]
PLC (basal)	[PIP2] * (PLC_basal + k_ PLC * fold PIP2 * Ga_GTP_PLC), where PLC_basal = 0.0001 s−1, k_PLC = 0.2 µm2molecule−1s−1 and fold PIP2 = 3
PLC (stimulated)	[PIP2] * (PLC_basal + k_PLC * fold PIP2 * Ga_GTP_PLC) + [PIP2] * PLC_stim * Ga_GTP_PLC * [Ca_C/(Ca_C + Kd_PLC_Ca)], where PLC_basal = 0.0001 s−1, k_PLC = 0.2 µm2molecule−1s−1, fold PIP2 = 3, PLC_stim = 7 s−1, and Kd_PLC_Ca = 1 µM
RL to RLP	kf_RL * [RL] * (weighting_factor_PKC_DAG * [PKC_DAG] + weighting_factor_GRK * [GRK]) − kr_RLP * [RLP]
PKC to active PKC	kf_PKC_DAG * [PKC] * [DAG] − kr_PKC_DAG * [PKC_DAG]
R to RP	kf_R * [R] * [PKC_DAG] − kr_RP * [RP]
RP to RLP	kf_L2 * [RP] − kr_L2 * [RLP]
RLP to RLPA1	kf_RLP * [RLP] * [Arrestin1] − kr_RLPA1 * [RLPA1]
RLP to RLPA2	kf_RLP * [RLP] * [Arrestin2] − kr_RLPA2 * [RLPA2]

Many additional reactions that have been already described previously in our paper are not in this table. The parameters are in previous papers (Dickson et al., 2013; Falkenburger et al., 2013). We added Ca²⁺-sensitive PLC activation in our model. In this table and calculation, the units of concentration and area are µM and µm², respectively.