Table 1.
Terminals | Kd,e1 | BT,e1 | Kd,e2 | BT,e2 | VA | [Ca2+]Free,0 | n |
µM | µM | µM | µM | µM | |||
One buffer | 1.5 ± 0.2 | 170 ± 46 | - | - | 0.52 ± 0.10 | 0.607 ± 0.13 | 8 |
Two buffer | 0.38 ± 0.08 | 340 ± 110 | 4.7 ± 0.8 | 140 ± 40 | 0.38 ± 0.05 | 0.570 ± 0.09 | 9 |
Properties of endogenous Ca2+ buffers in nerve terminals with one or two species. Kd and BT are the dissociation constant and total concentration of endogenous buffers, respectively (Eq. 2). VA is the fraction of total cellular volume accessible to Ca2+; [Ca2+]Free,0 is the resting Ca2+ concentration computed from resting F with Eq. 1.