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. 2016 Mar 1;6:22487. doi: 10.1038/srep22487

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Copyright © 2016, Macmillan Publishers Limited

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Huh7.5.1 cells were transfected with 25 nM small interference RNA (siRNA) against JNK, IRE1, ATF6 or PERK. After 48 hours of transfection, JNK, IRE1, ATF6, and PERK mRNA levels were determined by real-time PCR and normalized to GAPDH. The ON-TARGET plus SMART pool siRNAs had stronger mRNA knockdown effects for JNK, IRE1, ATF6, or PERK compared to its individual siRNA (Fig. 6A–D).