Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 1;6:22487. doi: 10.1038/srep22487

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Huh7.5.1 cells and Huh7.5.1 cells infected with JFH1 for 3 days were transfected with 25 nM small interference RNA (siRNA) against JNK, IRE1, ATF6, and PERK for 48 hours. The mRNA expression levels of TGF-β1, spliced XBP-1, ATF3, and ATF4 were determined by real-time PCR and normalized to GAPDH. We found that JNK siRNA or IRE1 siRNA blunted HCV-induced TGF-β1 and XBP-1s mRNA enhancement in JFH1-infected Huh7.5.1 cells (Fig. 8A,B). JNK siRNA or PERK siRNA decreased HCV-induced ATF3 and ATF4 mRNA expression enhancement in JFH1-infected Huh7.5.1 cells (Fig. 8C,D). *p < 0.05 for comparison of indicated siRNA-mediated knockdown and JFH1-infected cells.