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. 2016 Mar 1;6:22341. doi: 10.1038/srep22341

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(a) TNFα down-regulates eNOS transcript expression in HAoECs. Calcitriol increased the ability of MAC-conditioned media (MAC-D) to partially inhibit this down-regulation whilst MACs cultured without vitamin D had no significant effect (MAC). (b) Representative immunoblot of the change in eNOS expression in HAoEC. (c) eNOS protein expression shows the same pattern as the transcript with greatest effect with vitamin D-treated MACs but no effect of calcitriol in isolation. (a,c) show results from n = 3 and n = 4 patients respectively. *P < 0.05, **P < 0.01 by ratio paired t tests.