Figure 4. TCD induced reactive oxygen species (ROS) generation in MCF-7 cells.
(A) Cells were treated with DMSO or 20 μM TCD with or without glutathione (GLU) for 72 h. (B) Histogram of ROS production in MCF-7 cells treated with DMSO or TCD with or without GLU for 72 h. (n = 3). *P < 0.05; **P < 0.01. (C) Pre-treatment with 500 μM glutathione (GLU) rescued partially the apoptosis induced by TCD (20 μM) for 72 h (n = 3).