Skip to main content
. 2016 Mar 1;6:22419. doi: 10.1038/srep22419

Figure 5. TCD induced autophagy in MCF-7 cells.

Figure 5

(A) Electron microscopic analysis of autophagosome formation (arrow) in vehicle- or TCD-treated MCF-7 cells. Magnification, 12,000× (B) Fluorescent confocal microscopic analysis of TCD-induced autophagosome formation in MCF-7 cells ectopically expressing GFP-LC3. MCF-7 cells transfected with GFP-LC3 plasmids were treated with DMSO, 20 or 25 μM TCD, or 200 nM rapamycin for 30 min and then fixed by 3.7% paraldehyde and examined by confocal microscopy. The arrow indicates LC3-positive puncta in cells. (C) Dose- and time dependent effects of TCD on the expression of LC3B-II. (D) The apoptosis of MCF-7 cells induced by TCD could not be rescued by two autophagy inhibitors, chloroquine (CQ) and bafilomycin A1 (BA). MCF-7 cells were treated with DMSO, or TCD (15 μM) with or without 10 μM CQ or 5 nM BA for 72 h. (E) Histogram of autophagy inhibitors to increase the apoptotic cell percentage of MCF-7 induced by TCD. MCF-7 cells were treated with DMSO, or TCD (15 μM) with or without treatment of 10 μM CQ or 5 nM BA for 72 h (n = 3). *P < 0.05.