Figure 8. MAGL inhibition represses Aβ accumulation and neurodegeneration in response to NO₂ inhalation in C57BL/6J mice.

C57BL/6J mice were exposed to 5 mg/m³ NO₂ for 5 h/day for 4 weeks in the absence or presence of MAGL inhibitor, JZL184 (8 mg/kg, i.p.), and the expression of APP and BACE1 in the cortex (a) and hippocampus (b) were determined. Data were expressed as means ± SE (n = 6 to 8 mice/group), *P < 0.05, **P < 0.01 vs vehicle control group or NO₂ treatment group. Blots were cropped in this figure, but the gels were run under the same experimental conditions, and full-length blots are presented in Supplementary Figure S5. (c) Accumulation and deposition of Aβ₄₂ (green) in the brain. (d) Degenerated neurons (FJC, a neurodegeneration marker, green) in the cortex and hippocampus of mice. Scale bars = 25 μm. Data were averaged from 4 sections per mice, and n = 4 to 5 mice/group; *P < 0.05, **P < 0.01 vs NO₂ treatment group. Veh = vehicle; NO₂ = 5 mg/m³ NO₂; NO₂ + JZL = 5 mg/m³ NO₂ + 8 mg/kg JZL184.