Fig. 1.

Time-dependent JNK1 activation by uni-axial cyclic mechanical stretching of HBSMCs. a HBSMCs were exposed to uni-axial stretch (15 % elongation) for the indicated periods. The activity of JNK1 was measured by Western blotting using an anti-phosphorylated JNK1 (Thr183/Tyr185) antibody, which recognizes the active form of JNK1 (phosphorylated JNK1; P-JNK). The same filter was immunoblotted with each of the specific antibodies to demonstrate the total amount of JNK1 (Total-JNK1). b Quantification of the activity of JNK1 at each time point after stretch application. JNK1 activity was quantified using densitometry. The results are shown as means ± SEM (n = 6). The data are normalized by the total protein amount, and the intensity at 0 min was set at 1.0. An * indicates p < 0.05 compared to the value at time 0