Figure 8.

ESCRT-III recruitment model. (A) Cartoon illustrating cytokinesis in the absence or presence of chromosome segregation defects. (B) Model for ESCRT-III recruitment during cytokinetic abscission and checkpoint signaling. CHMP4B, the main component of the ESCRT-III filaments, is recruited via two parallel arms, namely CEP55–ALIX or CEP55–ESCRT-I–ESCRT-II–CHMP6. This recruitment is essential for timing of cytokinetic abscission. In contrast, recruitment of the abscission checkpoint regulator CHMP4C relies on ALIX, indicating that ALIX represent a dual functionality as abscission regulator and checkpoint signaling node.