Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 29;212(5):545–560. doi: 10.1083/jcb.201507023

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Wang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Defective self-renewal of Fip200-deficient NSCs in vitro restored by p62 KO. (A) Primary (first) and secondary (second) neurosphere formation from Ctrl, Fip200^GFAP cKO, 2cKO, and p62 KO mice at P28. Representative phase contrast images. (B) Mean ± SEM of the number and size of primary and secondary neurospheres (n = 3 mice each). (C and D) Mean ± SEM of the percentage of TUNEL⁺ (C) and Ki67⁺ (D) cells in neurospheres from Ctrl, Fip200^GFAP cKO, 2cKO, and p62 KO mice at P28 by immunofluorescence (n = 3 mice each, >500 cells per mouse). (E–H) Immunofluorescence of p62 (E), LC3 (F), TAX1BP1 (G), NBR1 (H), Fip200 (I), and DAPI in neurospheres from Ctrl, Fip200^GFAP cKO, 2cKO, and p62 KO mice at P28. Mean ± SEM of p62 aggregates (E), LC3 puncta (F), TAX1BP1 aggregates (G), and NBR1 aggregates (H) per neurosphere cell. Boxed areas in F show more detail for LC3 puncta, marked by arrows in insets (n = 3 mice each, >200 cells from Ctrl, 2cKO, and p62 KO mice; 87 cells for Fip200^GFAP cKO mouse). Bars: (A) 100 µm; (E–I and F and insets) 20 µm. **, P < 0.01; ***, P < 0.001; NS, not significant.