Fig. 4.

Markers of developmental signaling pathways are normal within cells that have mutations which prevent/mimic globular domain histone H3 modifications. Wing imaginal discs with GFP-negative mutant clones generated using Ubx-FLP. a Merged images show the DNA marker DAPI in blue, Wg in magenta, and GFP⁺ and GFP⁻ regions demarcate histone wild-type cells and histone mutant cells, respectively. Grayscale images are the individual Wg channels. b Merged images show the nuclear marker DAPI in blue, Ubx in magenta, and GFP⁺ and GFP⁻ regions demarcate histone wild-type cells and histone mutant cells, respectively. Grayscale images are the individual Ubx channels. Note that Ubx is expressed in the peripodial membrane of wing discs, tracheal cells that are accidently attached to the wing discs, as well as in leg discs, allowing for a positive control for the staining