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. 2016 Mar 1;6:22486. doi: 10.1038/srep22486

Figure 4. HLA-DR4 binding assay.

Figure 4

(A) Binding activities between a HLA-DRB1*0401 and 0405 molecule and MycHSP70287–306. Binding activities were estimated by the inhibition of peroxidase-labeled HA307–318 peptide binding to a HLA-DRB1 molecule. (B) Binding of MycHSP70287–306 with alanine-substituted peptides to HLA-DRB1*0405 molecules. A MycHSP70287–306-derived peptide in which an amino residue was substituted by an alanine residue. Inhibitory concentration 50% (IC50) was used as a measure of binding activity. *p < 0.05, **p < 0.01, ***p < 0.001.