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. 2016 Mar 1;6:22486. doi: 10.1038/srep22486

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(A) HLA-DR4 transgenic mice were immunized with recombinant MycHSP70. Serum anti-MycHSP70 and anti-mouse BiP antibody titers were measured in the 21 days following the first immunization. (B) Splenic CD4+ T cell proliferation from MycHSP70-immunized HLA-DR4 transgenic mice in response to MycHSP70_287–306 and BiP_336–355 epitopes. Proliferation was determined by the incorporation of ³-H thymidine. Data were representative of at least three independent trials. ***p < 0.001.