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. 2016 Mar 1;6:22439. doi: 10.1038/srep22439

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Copyright © 2016, Macmillan Publishers Limited

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(a) The main cis-elements including one TCA-element, one HSE, one ERE and seven W-boxes in pCaCDPK15. (b) The pCaCDPK15 driven GUS expression was promoted by exogenous application of SA, MeJA, ETH and ABA and RSI. The leaves of pepper plants at eighty-leaf stage ware infiltrated with GV3101 cells (OD₆₀₀ = 0.6) containing pCabZIP63:GUS, and 24 hours later the plants were treated with 1 mm SA, 100 μm MeJA, 100 μm ETH, 100 μm ABA, or inoculated with the R. solanacearum (OD₆₀₀ = 0.6). The leaves were harvested at different time points for GUS activity assay by microplate reader using pepper leaves treated with ddH₂O as mock. Data are the means ± SD from at least three independent experiments. Asterisks indicate statistically significant differences compared with Mock (treated with ddH₂O). (t-test, **P < 0.01).