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. 2016 Mar 1;6:22439. doi: 10.1038/srep22439

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(a) The effect of transient overexpression of CaWRKY40 and CaWRKY40-SRDX on the transcript level of CaCDPK15 and immunity associated marker genes CaPR1, CaNPR1 and CaDEF1 in pepper leaves at 24 hpi. (b) The transcriptional expression of CaCDPK15 was downregulated significantly in CaWRKY40-silenced pepper plants inoculated by R. solanacearum after 24 hours. Expression values are normalized by the expression levels of CaACTIN and 18s rRNA. (a,b) Data are the means ± SD from at least three independent experiments. Asterisks indicate statistically significant differences compared with EV ([a]) and the treatment of MgCl₂ (Mock, [b]). (t-test, **P < 0.01).