FIG 3.

SSA promotes nitrate assimilation in A. tumefaciens. (A) The metabolic pathway for nitrate assimilation in A. tumefaciens. Glu, glutamate; Gln, glutamine. (B) Arrangement of nitrate assimilatory gene clusters and their induction profiles in microarray. The clusters are composed of the genes encoding putative nitrate reductase, nitrite reductase, glutamine synthetase, glutamate synthase, and related transporters and regulators (open columns). The arrows indicate the transcriptional direction. (C) Growth of A. tumefaciens using KNO₃ as the sole nitrogen source with or without SSA treatment. The fresh bacteria from LB agar were resuspended in PBS and then inoculated at a ratio of 1% in a defined medium. −, no chemical addition; +, chemical addition. Error bars denote standard deviations.