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AttKL, but not AttJ and AttM, was required for nitrate utilization in A. tumefaciens. Bacterial growth of A. tumefaciens A6 and its mutants was determined using KNO3 as the sole nitrogen source. OD600 values were recorded for cells cultured with shaking at 220 rpm at 28°C for 24 h. Open bars, no SSA added; filled bars, SSA added. Error bars denote standard deviations.
